IGDB.NSCLC Gene View
 
Gene Information        (help)
Gene GCLC Ensembl ENSG00000001084 Chromosome 6 Start 53470098 End 53517790
Description Glutamate--cysteine ligase catalytic subunit (EC 6.3.2.2)(Gamma-glutamylcysteine synthetase)(Gamma-ECS)(GCS heavy chain) [Source:UniProtKB/Swiss-Prot;Acc:P48506]
GENE RESOURCES :NUCLEOTIDE SEQUENCES :PROTEIN RESOURCES :CLINICAL RESOURCES :REFERENCES :
     HGNC : 4311
     Entrez Gene : 2729
     UCSC : uc003pbw.1
     GeneCards : 4311
     RefSeq : NM_001498
     CCDS : CCDS4952.1
     Uniprot : P48506
     Interpro : P48506
     OMIM : 606857
     GeneTests : GCLC
     CGAP : GCLC
     PMID : 1350904

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Microarray Gene Expression Fold Change Result        (help)
( red: up-regulation / green : down-regulation when p value < 0.01)
( gray background : these probesets might have mapping problems. ref 1, ref 2)
Chip Type Probeset Adenocarcinoma Squamous Cell Carcinoma
Fold Change p value q value Fold Change p value q value
 HG_U95  31850_at  1.29  5.69e-8  4.90e-7  2.31  6.24e-15  1.94e-13
 HG_U133A  202922_at  0.54  3.99e-6  9.68e-6  0.37  1.25e-7  1.50e-7
 HG_U133A  202923_s_at  1.07  4.93e-12  2.10e-11  0.91  2.15e-39  4.12e-39
 HG_U133_Plus2  1555330_at  0.82  4.07e-4  1.15e-3  1.99  1.86e-14  1.38e-13
 HG_U133_Plus2  202922_at  0.82  4.91e-8  2.72e-7  1.93  4.26e-26  1.19e-24
 HG_U133_Plus2  202923_s_at  1.35  1.03e-12  1.05e-11  2.57  9.78e-33  6.17e-31
 Stanford  3057  0.98  2.22e-2  1.08e-1  1.92  8.55e-5  2.46e-3
 Agilent_HS_21.6K  14771  0.14  3.99e-1  5.86e-1  0.79  4.59e-5  4.11e-4

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Adjuvant Cisplatin/vinorelbine Treatment vs Observation Result        (help) (Pubmed)
( red: up-regulation / green : down-regulation when p value < 0.01)
( gray background color : the mapping problems of probeset. ref_1, ref_2)
Chip Type Probeset Adenocarcinoma Squamous Cell Carcinoma
Fold Change p value q value Fold Change p value q value
 HG_U133A  202922_at  0.27  3.70e-1  8.96e-1  -0.18  5.34e-1  1.00e+0
 HG_U133A  202923_s_at  0.91  5.14e-2  7.08e-1  -0.38  3.43e-1  1.00e+0

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Microarray Sample Data        (help)
( The log2 value of tumor samples )
(Average : Average log2 value from Normal Samples.)
        HG_U95 - 31850_at    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        HG_U133A - 202922_at    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        HG_U133A - 202923_s_at    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        HG_U133_Plus2 - 1555330_at    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        HG_U133_Plus2 - 202922_at    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        HG_U133_Plus2 - 202923_s_at    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        Stanford - 3057    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

        Agilent_HS_21.6K - 14771    (back)       Save as a PNG file. Save as a PDF file. Save as a PS file.
Gene expression figure

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Cancer Gene Index        (help)

If 0 entry was found, please remove the search key "lung cancer".
Keyword DiseaseData Statement PubMed Organism
gamma-glutamylcysteine synthetase human colon cancer Induction of multidrug resistance proteins MRP1 and MRP3 and gamma-glutamylcysteine synthetase gene expression by nonsteroidal anti-inflammatory drugs in human colon cancer cells. 11820781 Human
gamma-glutamylcysteine synthetase primary lung cancer METHODS: We studied DNA polymorphisms of 250 primary lung cancer patients at four glutathione-related loci: GSTP1, GSTM1, GSTT1 and gamma-GCS that encode glutathione-S-transferase-pi, glutathione-S-transferase-mu, glutathione-S-transferase-theta, and gamm 11844594 NA
gclc hepatoma In mouse Hepa-1c1c7 hepatoma cell cultures, we found that tert-butylhydroquinone (tBHQ; 50 microM) induces the GCLM and GCLC mRNAs approximately 10- and approximately 2-fold, respectively, and that these increases primarily reflect de novo transcription. 12007577 Mouse
gamma-glutamylcysteine synthetase tumors To investigate possible mechanisms of this protective activity, we evaluated by RT-PCR the gene expression of cycloxygenase-2 (COX-2), inducible NO synthase (iNOS), gamma-glutamylcysteine synthetase (gamma-GCS) and two isoforms of glutathione S-transferas 12042461 Human
gamma-glutamylcysteine synthetase glioblastoma Hammerhead ribozyme against gamma-glutamylcysteine synthetase attenuates resistance to ionizing radiation and cisplatin in human T98G glioblastoma cells. 12079521 Human
gamma-glutamylcysteine synthetase glioblastoma In this study, the effect of continuous down-regulation of gamma-glutamylcysteine synthetase (gamma-GCS) expression, a rate-limiting enzyme for GSH synthesis, on resistance to ionizing radiation and cisplatin (CDDP) was studied in T98G human glioblastoma 12079521 NA
gamma-glutamylcysteine synthetase human malignant mesothelioma Overexpression of gamma-glutamylcysteine synthetase in human malignant mesothelioma. 12196927 Human
gcs malignant mesothelioma However, the expression of this enzyme has not been systematically investigated in malignant tumors, and there are no studies of gamma GCS in biopsy specimens of malignant mesothelioma. 12196927 Human
gcs mesothelioma We investigated the immunohistochemical distribution and expression of both subunits of gamma GCS in healthy pleural mesothelium, pleural mesothelioma tumor biopsy samples (34 cases), and mesothelioma cells in culture (7 cell lines). 12196927 Human
gcs tumor We investigated the immunohistochemical distribution and expression of both subunits of gamma GCS in healthy pleural mesothelium, pleural mesothelioma tumor biopsy samples (34 cases), and mesothelioma cells in culture (7 cell lines). 12196927 Human
gcs pleural mesothelioma We investigated the immunohistochemical distribution and expression of both subunits of gamma GCS in healthy pleural mesothelium, pleural mesothelioma tumor biopsy samples (34 cases), and mesothelioma cells in culture (7 cell lines). 12196927 Human
gcs tumors High-moderate and weak immunoreactivity of the light (regulatory) subunit of gamma GCS was found in 15 and 7 tumors, respectively, whereas 12 cases showed no reactivity. 12196927 Human
gcs mesothelioma Treatment of cultured mesothelioma cells with buthionine sulfoximine (BSO), to inhibit gamma GCS, significantly potentiated cisplatin-induced cytotoxicity mainly by nonapoptotic mechanism when assessed by counting the living cells, TUNEL (terminal deoxytr 12196927 Human
gcs tumor In conclusion, gamma GCS is highly positive in most cases of malignant mesothelioma and may play an important role in the primary drug resistance of this tumor in vivo. 12196927 Human
gcs malignant mesothelioma In conclusion, gamma GCS is highly positive in most cases of malignant mesothelioma and may play an important role in the primary drug resistance of this tumor in vivo. 12196927 Human
gamma-glutamylcysteine synthetase human colorectal carcinoma Differential sensitivities of the MRP gene family and gamma-glutamylcysteine synthetase to prooxidants in human colorectal carcinoma cell lines with different p53 status. 11239498 Human
gamma-glutamylcysteine synthetase hepatocellular carcinomas Transaldolase, rat aflatoxin B1 aldehyde reductase and gamma-glutamylcysteine synthetase were found as up-regulated genes and regucalcin as a down-regulated gene, in line with findings for hepatocellular carcinomas. 14656948 Rat
gamma-glutamylcysteine synthetase colonic cancer Hammerhead ribozyme against gamma-glutamylcysteine synthetase sensitizes human colonic cancer cells to cisplatin by down-regulating both the glutathione synthesis and the expression of multidrug resistance proteins. 11687904 Human
gamma-glutamylcysteine synthetase cancer Multidrug resistance in cancer cells is often associated with an elevation in the concentration of glutathione (GSH) and the expression of gamma-glutamylcysteine synthetase (gamma-GCS), a rate-limiting enzyme for GSH. 11687904 Human
gcs tumors The MRP5 mRNA levels in tumors from lung cancer patients treated with platinum regimen were significantly higher than in tumors from patients treated with non-platinum regimens, and the MRP5 expression levels were correlate with the GCS expression levels 11766988 Human
gcs lung cancer The MRP5 mRNA levels in tumors from lung cancer patients treated with platinum regimen were significantly higher than in tumors from patients treated with non-platinum regimens, and the MRP5 expression levels were correlate with the GCS expression levels 11766988 Human
gcs nonsmall-cell lung carcinoma (nsclc) BACKGROUND: The purpose of this study was to investigate expression of gamma glutamyl cysteine synthetase (gamma GCS), the rate-limiting enzyme in glutathione synthesis in nonsmall cell lung carcinoma (NSCLC). 11753966 Human
gcs tumor CONCLUSIONS: The results show that gamma GCS is strongly expressed in NSCLCs and probably takes part in the defense of the tumor cells against oxidative damage. 11753966 Human
gcs tumors This is reflected by the lower extent of apoptosis in tumors with a high gamma GCS expression. 11753966 Human
gcs lung carcinoma Because expression of gamma GCS has been connected with chemoresistance, downregulation of its activity by inhibitors in NSCLC might have putative therapeutic potential in the treatment of lung carcinoma. 11753966 Human
gamma-glutamylcysteine synthetase lung cancer Carboplatin AUC and gamma-glutamylcysteine synthetase gene expression in peripheral mononuclear cells of lung cancer patients. 11911273 Human
gamma-glutamylcysteine synthetase lung cancer BACKGROUND: To investigate the association between glutathione-related enzymes and carboplatin (CBDCA) dose, we examined gene expression levels for both subunits of gamma-glutamylcysteine synthetase (heavy; gamma-GCSh, light; gamma-GCS1) in peripheral mon 11911273 Human
gamma-glutamylcysteine synthetase tumors Documented increases in gamma-glutamylcysteine synthetase (GCS) occur in response to oxidants, in tumors, on plating cells at a low cell density, and with nerve growth factor stimulation, suggesting that GSH synthesis may be related to the cell growth and 10683268 Human
gcs tumors Documented increases in gamma-glutamylcysteine synthetase (GCS) occur in response to oxidants, in tumors, on plating cells at a low cell density, and with nerve growth factor stimulation, suggesting that GSH synthesis may be related to the cell growth and 10683268 Human
gamma-glutamylcysteine synthetase human promyelocytic leukemia Although glutathione levels were elevated in human promyelocytic leukemia/TER286 cells, no changes in the expression of thiol-related genes including gamma-glutamylcysteine synthetase, gamma-glutamyl transpeptidase, or multidrug resistance protein were fo 10860939 Human
gamma-glutamylcysteine synthetase human colorectal cancer Induction of MRP1 and gamma-glutamylcysteine synthetase gene expression by interleukin 1beta is mediated by nitric oxide-related signalings in human colorectal cancer cells. 11025451 Human
gamma-glutamylcysteine synthetase human colorectal cancer Treatment of human colorectal cancer cells HT29 with interleukin 1beta (IL-1beta) induces expression of the multidrug resistance protein (MRP1) gene encoding the ATP-dependent glutathione S-conjugate export (GS-X) pump and the gamma-glutamylcysteine synth 11025451 Human
gamma-glutamylcysteine synthetase colorectal cancer Genes encoding both MRP1 and the catalytic subunit of gamma-glutamylcysteine synthetase (gamma-GCS) are coordinately regulated in cultured cancer cell lines as well as colorectal cancer tissues from colon cancer patients. 11097346 Human
gamma-glutamylcysteine synthetase colon cancer Genes encoding both MRP1 and the catalytic subunit of gamma-glutamylcysteine synthetase (gamma-GCS) are coordinately regulated in cultured cancer cell lines as well as colorectal cancer tissues from colon cancer patients. 11097346 Human
gamma-glutamylcysteine synthetase cancer Genes encoding both MRP1 and the catalytic subunit of gamma-glutamylcysteine synthetase (gamma-GCS) are coordinately regulated in cultured cancer cell lines as well as colorectal cancer tissues from colon cancer patients. 11097346 Human
gamma-glutamylcysteine synthetase acute myeloblastic leukemia Protection of acute myeloblastic leukemia cells against apoptotic cell death by high glutathione and gamma-glutamylcysteine synthetase levels during etoposide-induced oxidative stress. 10631466 Human
gamma-glutamylcysteine synthetase human colorectal carcinoma Expression of heavy subunit of gamma-glutamylcysteine synthetase (gamma-GCSh) in human colorectal carcinoma. 11774239 Human
gamma-glutamylcysteine synthetase lung cancer Induction of gamma-glutamylcysteine synthetase gene expression by platinum drugs in peripheral mononuclear cells of lung cancer patients. 10370789 Human
gamma-glutamylcysteine synthetase cancer Co-expression of gamma-glutamylcysteine synthetase sub-units in response to cisplatin and doxorubicin in human cancer cells. 10399958 Human
gamma-glutamylcysteine synthetase human malignant mesothelioma Co-ordinated over-expression of the MRP and gamma-glutamylcysteine synthetase genes, but not MDR1, correlates with doxorubicin resistance in human malignant mesothelioma cell lines. 9495245 Human
gamma-glutamylcysteine synthetase mesothelioma In this study, we used normal human mesothelial cells and 5 human mesothelioma cell lines not previously exposed to chemotherapeutic agents to demonstrate that the mRNA for the multidrug resistance-associated protein (MRP) and gamma-glutamylcysteine synth 9495245 NA
gamma-glutamylcysteine synthetase tumor Frequent coexpression of MRP/GS-X pump and gamma-glutamylcysteine synthetase mRNA in drug-resistant cells, untreated tumor cells, and normal mouse tissues. 9515571 Human
gamma-glutamylcysteine synthetase human colorectal tumors We have shown recently that the MRP and gamma-glutamylcysteine synthetase (gamma-GCS) heavy subunit mRNA levels are coordinately overexpressed in cisplatin (CP)-resistant human leukemia cells (Ishikawa et al., J Biol Chem 271: 14981-14988, 1996) and frequ 9515571 Human
gamma-glutamylcysteine synthetase human leukemia We have shown recently that the MRP and gamma-glutamylcysteine synthetase (gamma-GCS) heavy subunit mRNA levels are coordinately overexpressed in cisplatin (CP)-resistant human leukemia cells (Ishikawa et al., J Biol Chem 271: 14981-14988, 1996) and frequ 9515571 Human
gamma-glutamylcysteine synthetase human lung cancers Expression of gamma-glutamylcysteine synthetase (gamma-GCS) and multidrug resistance-associated protein (MRP), but not human canalicular multispecific organic anion transporter (cMOAT), genes correlates with exposure of human lung cancers to platinum drug 9569044 Human
gamma-glutamylcysteine synthetase human lung cancer We examined the steady-state levels of mRNA for gamma-glutamylcysteine synthetase (gamma-GCS), multidrug resistance-associated protein (MRP) and human canalicular multispecific organic anion transporter (cMOAT) in human lung cancer specimens to elucidate 9569044 NA
gamma-glutamylcysteine synthetase glioblastoma J. Biol. Chem., 1996). Here, we present direct evidence that NF-kappa B activated by ionizing radiation induces the expression of gamma-glutamylcysteine synthetase (gamma-GCS), the rate limiting enzyme of GSH synthesis, using T98G human glioblastoma cells 9626582 Human
gamma-glutamylcysteine synthetase colorectal cancer Phase 1 clinical evaluation of oltipraz has demonstrated its ability to induce GST activity as well as the level of transcripts encoding gamma-glutamylcysteine synthetase (gamma-GCS) and DT-diaphorase in the colon mucosa of individuals at increased risk f 9679568 Human
gamma-glutamylcysteine synthetase rectum cancer In this study, lipid peroxide and glutathione (GSH) levels, GSH peroxidase, GSH S-transferase, superoxide dismutase, gamma-glutamylcysteine synthetase and gamma-glutamyl transpeptidase activities were investigated in tumorous and nontumorous colorectal ti 9829859 NA
gamma-glutamylcysteine synthetase colorectal cancer According to the results, lipid peroxide and GSH levels and the activities of GSH peroxidase, superoxide dismutase, gamma-glutamylcysteine synthetase were found to be increased, while GSH S-transferase and gamma-glutamyl transpeptidase activities remained 9829859 Human
gamma-glutamylcysteine synthetase tumors According to the results, lipid peroxide and GSH levels and the activities of GSH peroxidase, superoxide dismutase, gamma-glutamylcysteine synthetase were found to be increased, while GSH S-transferase and gamma-glutamyl transpeptidase activities remained 9829859 Human
gamma-glutamylcysteine synthetase bladder cancer Enhanced expression of gamma-glutamylcysteine synthetase and glutathione S-transferase genes in cisplatin-resistant bladder cancer cells with multidrug resistance phenotype. 9072542 Human
gamma-glutamylcysteine synthetase cancer Effect of glutathione depletion on cisplatin resistance in cancer cells transfected with the gamma-glutamylcysteine synthetase gene. 9119737 Human
gamma-glutamylcysteine synthetase human ovarian cancer Altered expression of gamma-glutamylcysteine synthetase, metallothionein and topoisomerase I or II during acquisition of drug resistance to cisplatin in human ovarian cancer cells. 9119751 Human
gcs neck squamous cancer PURPOSE: To correlate cellular glutathione content and gamma-glutamyl cysteine synthetase (gamma GCS) mRNA expression with cisplatin sensitivity in a panel of seven head and neck squamous cancer cell lines. 9137534 NA
gcs ovarian cancer Some-what surprisingly, in contrast to previous studies in ovarian cancer, gamma GCS mRNA expression in these cell lines was not significantly predictive of either total cellular glutathione or cisplatin sensitivity (R2 = 0.005, P = 0.84). 9137534 Human
gamma-glutamylcysteine synthetase breast tumor In this study, using the estrogen receptor (ER)-positive human breast tumor cell line MCF-7, we have shown that 10 microM L-buthionine-[S,R]-sulfoximine (L-BSO), an inhibitor of gamma-glutamylcysteine synthetase (the rate-limiting enzyme in glutathione sy 9157984 NA
gamma-glutamylcysteine synthetase adult soft tissue sarcoma As treatment with chemotherapy has added little to improved survival in adult soft tissue sarcoma and little is known concerning the mechanisms of chemoresistance in sarcoma, we studied concentrations of glutathione (nmol/mg protein) and activities of gam 9181229 Human
gcs adult soft tissue sarcoma As treatment with chemotherapy has added little to improved survival in adult soft tissue sarcoma and little is known concerning the mechanisms of chemoresistance in sarcoma, we studied concentrations of glutathione (nmol/mg protein) and activities of gam 9181229 Human
gamma-glutamylcysteine synthetase soft-tissue sarcoma As treatment with chemotherapy has added little to improved survival in adult soft tissue sarcoma and little is known concerning the mechanisms of chemoresistance in sarcoma, we studied concentrations of glutathione (nmol/mg protein) and activities of gam 9181229 Human
gcs soft-tissue sarcoma As treatment with chemotherapy has added little to improved survival in adult soft tissue sarcoma and little is known concerning the mechanisms of chemoresistance in sarcoma, we studied concentrations of glutathione (nmol/mg protein) and activities of gam 9181229 Human
gamma-glutamylcysteine synthetase sarcoma As treatment with chemotherapy has added little to improved survival in adult soft tissue sarcoma and little is known concerning the mechanisms of chemoresistance in sarcoma, we studied concentrations of glutathione (nmol/mg protein) and activities of gam 9181229 Human
gcs sarcoma As treatment with chemotherapy has added little to improved survival in adult soft tissue sarcoma and little is known concerning the mechanisms of chemoresistance in sarcoma, we studied concentrations of glutathione (nmol/mg protein) and activities of gam 9181229 Human
gcs tumors While GCS activity was lower in low-grade (0.81 +/- 0.3 nmol/mg protein/h) than high-grade (1.49 +/- 0.5 nmol/mg protein/h) tumors and pulmonary metastases (1.03 +/- 0.2 nmol/mg protein/h), these differences were not significant. 9181229 Human
gcs pulmonary metastases While GCS activity was lower in low-grade (0.81 +/- 0.3 nmol/mg protein/h) than high-grade (1.49 +/- 0.5 nmol/mg protein/h) tumors and pulmonary metastases (1.03 +/- 0.2 nmol/mg protein/h), these differences were not significant. 9181229 Human
gamma-glutamylcysteine synthetase squamous carcinoma Inhibition by 5-fluorouracil of ERCC1 and gamma-glutamylcysteine synthetase messenger RNA expression in a cisplatin-resistant HST-1 human squamous carcinoma cell line. 9268987 Human
gcs cancer Therefore, elevation of GSH as achieved by inducing g-glutamylcysteine synthetase (GCS), the limiting enzyme of GSH synthesis, may contribute to chemoprevention against cancer. 12791507 Human
gcs hepatoma In the present experiment, we used the hepatoma cell line HepG2 to investigate the response of GCS and GSH to five plant-derived chemoprotectants contained in regularly consumed foodstuffs and beverages (kahweol/cafestol [K/C] [15.5-62.0 mM], a-angelicala 12791507 Human
gamma-glutamylcysteine synthetase glioma Posttranscriptional regulation of MRP/GS-X pump and gamma-glutamylcysteine synthetase expression by 1-(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethyl)-3-nitrosourea and by cycloheximide in human glioma cells. 9345268 Human
gamma-glutamylcysteine synthetase glioma Treatment of human glioma A172 cells with 1-(4-amino-2-methyl-5-pyrimidinyl) methyl-3-(2-chloroethy-3-nitrosourea (ACNU) for 2 to 4 hr resulted in a 2- to 3-fold increase in steady-state levels of multidrug resistance-associated protein (MRP) and gamma-gl 9345268 Human
gamma-glutamylcysteine synthetase tumor Pretreatment by L-buthionine sulfoximine (BSO), which inactivates gamma-glutamylcysteine synthetase and, therefore, inhibits glutathione (GSH) synthesis, greatly increased the sensitivity of tumor cells to the antiproliferative effects of several NO-donat 8694844 Human
gamma-glutamylcysteine synthetase ovarian carcinoma In a series of ovarian carcinoma cell lines selected in vitro for resistance to cisplatin by continuous exposure to increasing drug concentrations, the level of resistance is proportional to the expression of gamma-glutamylcysteine synthetase (gamma-GCS). 8678904 Human
gamma-glutamylcysteine synthetase human leukemia Coordinated induction of MRP/GS-X pump and gamma-glutamylcysteine synthetase by heavy metals in human leukemia cells. 8663001 Human
gamma-glutamylcysteine synthetase human colorectal cancers Frequent coordinated overexpression of the MRP/GS-X pump and gamma-glutamylcysteine synthetase genes in human colorectal cancers. 8705999 Human
gamma-glutamylcysteine synthetase human leukemia We have recently shown that multidrug resistance-associated protein (MRP) and gamma-glutamylcysteine synthetase (gamma-GCS) heavy subunit genes are coordinately overexpressed in cisplatin-resistant human leukemia cells (T. 8705999 Human
gamma-glutamylcysteine synthetase lung cancer gamma-Glutamylcysteine synthetase in lung cancer. 12415696 Human
gamma-glutamylcysteine synthetase tumor Increase in the reduced glutathione concentration was preceded by significant increase in the oxidized glutathione as well as in the activities of gamma-glutamylcysteine synthetase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, 9095465 Rat
gamma-glutamylcysteine synthetase human ovarian carcinoma Our previous studies have shown that sublines of human ovarian carcinoma cell line A2780, which exhibited low levels of resistance to oxaliplatin, showed elevated steady state levels of mRNA and activity of gamma-glutamyl transpeptidase (gamma-GT, EC 2.3. 9114434 Human
gamma-glutamylcysteine synthetase tumors The administration of buthionine sulfoximine (BSO), an irreversible inhibitor of gamma-glutamylcysteine synthetase, produces glutathione (GSH) depletion in tumors, making them sensitive to drugs and radiation. 7632167 Human
gamma-glutamylcysteine synthetase human ovarian cancer Evidence for altered regulation of gamma-glutamylcysteine synthetase gene expression among cisplatin-sensitive and cisplatin-resistant human ovarian cancer cell lines. 7671249 Human
gamma-glutamylcysteine synthetase tumor We have shown previously that tumor cell resistance to cisplatin is associated with elevated intracellular levels of glutathione, which is accomplished at least in part by increased expression of the heavy subunit of gamma-glutamylcysteine synthetase (gam 7671249 Human
gcs tumor Liver GCS activity (nanomolar per hour per milligram protein) in TB rats (106.6 +/- 37.7) was increased (p < 0.01) compared with CTL rats (57.5 +/- 12.3) and correlated positively with tumor burden (R = 0.77). 7552623 Rat
gcs tumor Liver GCS is increased in TB rats and skeletal muscle GGTP is decreased, which may preferentially benefit the tumor by increasing the bioavailability of glutathione for its own use. 7552623 Rat
gamma-glutamylcysteine synthetase tumor We cotransfected COS cells with the cDNAs for the two subunits of gamma-glutamylcysteine synthetase (GCS), which catalyzes the rate-limiting step in the de novo synthesis of GSH and is itself up-regulated in some drug-resistant tumor cells. 7585502 Human
gcs tumor We cotransfected COS cells with the cDNAs for the two subunits of gamma-glutamylcysteine synthetase (GCS), which catalyzes the rate-limiting step in the de novo synthesis of GSH and is itself up-regulated in some drug-resistant tumor cells. 7585502 Human
gamma-glutamylcysteine synthetase small-cell lung cancer We transfected the human gamma-glutamylcysteine synthetase (gamma-GCS) gene into a human small-cell lung cancer cell line, SBC-3, producing SBC-3/GCS. 7488097 Human
gamma-glutamylcysteine synthetase hepatoma In this paper the in vitro activities of two enzymes involved in glutathione synthesis, gamma-glutamylcysteine synthetase and glutathione synthetase, are studied in normal adult rat liver, in regenerating rat liver and in highly anaplastic Yoshida AH-130 3809895 Human
gamma-glutamylcysteine synthetase hepatoma In Yoshida AH-130 hepatoma, the gamma-glutamylcysteine synthetase and glutathione synthetase activities are lower in respect to normal liver. 3809895 Rat
gamma-glutamylcysteine synthetase tumor Since the inhibitory effects of Cys on both the decrease in the ratio of GSH/GSSG and the induction of ODC activity by TPA were greatly reduced by the inhibitors of gamma-glutamyl transpeptidase and gamma-glutamylcysteine synthetase, it is suggested that 2859127 Human
gamma-glutamylcysteine synthetase tumor The importance of GSH resynthesis upon exposure of tumor cells to SL was evaluated with the use of buthionine sulfoximine (BSO), a selective, nontoxic inhibitor of gamma-glutamylcysteine synthetase. 6401768 Human
gamma-glutamylcysteine synthetase tumor These results suggest that the drug-resistant tumor cell has developed an efficient mechanism for maintenance of elevated GSH which involves both gamma-glutamyl transpeptidase-initiated catabolism of GSH to cysteine and its reutilization by gamma-glutamyl 3667623 Mouse
gamma-glutamylcysteine synthetase melanomas We have studied the effect of buthionine sulfoximine (BSO; a gamma-glutamylcysteine synthetase inhibitor) administration, either alone or combined with misonidazole (MISO), on five human tumor xenografts (three melanomas: Bell, Mall, and Nall; and two rec 3945723 NA
gamma-glutamylcysteine synthetase human tumor We have studied the effect of buthionine sulfoximine (BSO; a gamma-glutamylcysteine synthetase inhibitor) administration, either alone or combined with misonidazole (MISO), on five human tumor xenografts (three melanomas: Bell, Mall, and Nall; and two rec 3945723 NA
gamma-glutamylcysteine synthetase adenocarcinomas We have studied the effect of buthionine sulfoximine (BSO; a gamma-glutamylcysteine synthetase inhibitor) administration, either alone or combined with misonidazole (MISO), on five human tumor xenografts (three melanomas: Bell, Mall, and Nall; and two rec 3945723 NA
gamma-glutamylcysteine synthetase cancer The decrease in GSH concentration within the cancer cells is associated with a decrease in gamma-glutamylcysteine synthetase activity and in protein synthesis. 8129737 Mouse
gamma-glutamylcysteine synthetase multiple myeloma Up-regulation of gamma-glutamylcysteine synthetase activity in melphalan-resistant human multiple myeloma cells expressing increased glutathione levels. 7513621 Human
gamma-glutamylcysteine synthetase multiple myeloma Levels of intracellular glutathione (GSH) and the GSH-related enzymes gamma-glutamylcysteine synthetase (gamma-GCS) and gamma-glutamyltranspeptidase (gamma-GT) were measured in the melphalan-resistant human multiple myeloma cell line 8226/LR-5 and were co 7513621 Human
gcs refractory tumor Since GCS catalyzes the rate-limiting step of GSH synthesis, increased activity of this enzyme may be one mechanism by which intracellular GSH levels may be upregulated in treatment refractory tumor cells. 7910817 Human
gamma-glutamylcysteine synthetase human prostate carcinoma Transcriptional up-regulation of gamma-glutamylcysteine synthetase gene expression in melphalan-resistant human prostate carcinoma cells. 7969079 Human
gcs human prostate carcinoma Using melphalan-resistant DU 145/M4.5 human prostate carcinoma cells, which express elevated GSH levels, GCS enzyme activity, and GCS mRNA levels, we sought to determine the mechanism(s) responsible for the increased GCS mRNA expression. 7969079 Human
gcs tumor These data suggest that GCS expression is transcriptionally regulated in these melphalan-resistant tumor cells. 7969079 Human
gamma-glutamylcysteine synthetase cancer Variable baseline gamma-glutamylcysteine synthetase messenger RNA expression in peripheral mononuclear cells of cancer patients, and its induction by buthionine sulfoximine treatment. 8101766 Human
gamma-glutamylcysteine synthetase ovarian cancer The rate-limiting enzyme in GSH synthesis is gamma-glutamylcysteine synthetase (gamma-GCS), the expression of which is proportional both to GSH content and to the level of resistance in ovarian cancer cell lines. 8101766 Human
gamma-glutamylcysteine synthetase malignant astrocytoma Purification and biochemical characterization of gamma-glutamylcysteine synthetase from a human malignant astrocytoma cell line. gamma-Glutamylcysteine synthetase (EC 6.3.2.2.) the key regulatory enzyme in glutathione biosynthesis was purified from a huma 8106072 Human
gamma-glutamylcysteine synthetase astrocytoma These molecular and catalytic properties of gamma-glutamylcysteine synthetase from astrocytoma cell line are similar, but not identical to those purified from rat kidney. 8106072 Human
gamma-glutamylcysteine synthetase human prostate carcinoma Increase in gamma-glutamylcysteine synthetase activity and steady-state messenger RNA levels in melphalan-resistant DU-145 human prostate carcinoma cells expressing elevated glutathione levels. 1355406 Human
gamma-glutamylcysteine synthetase human prostate carcinoma In a series of L-phenylalanine mustard-resistant human prostate carcinoma cell lines (DU-145), resistance was associated with elevated GSH levels, increased activity of gamma-glutamylcysteine synthetase (GCS), the rate-limiting enzyme in GSH biosynthesis, 1355406 Human
gcs human prostate carcinoma In a series of L-phenylalanine mustard-resistant human prostate carcinoma cell lines (DU-145), resistance was associated with elevated GSH levels, increased activity of gamma-glutamylcysteine synthetase (GCS), the rate-limiting enzyme in GSH biosynthesis, 1355406 Human
gclc non-small cell lung cancer GCLM is a potentially more effective pharmacologic target for ameliorating CDDP-resistance in non-small cell lung cancer than GCLC. 14532974 Human
gclc non-small cell lung cancer We examined the gene expression of GCLM and GCLC in 39 xenografts of human non-small cell lung cancer [NSCLC; 10 adenocarcinoma (Ad), 17 squamous cell carcinoma (Sq) and 12 large cell carcinoma (La)] by real-time polymerase chain reaction (PCR) with human 15254739 NA
gclc adenocarcinoma We examined the gene expression of GCLM and GCLC in 39 xenografts of human non-small cell lung cancer [NSCLC; 10 adenocarcinoma (Ad), 17 squamous cell carcinoma (Sq) and 12 large cell carcinoma (La)] by real-time polymerase chain reaction (PCR) with human 15254739 NA
gclc nsclc We examined the gene expression of GCLM and GCLC in 39 xenografts of human non-small cell lung cancer [NSCLC; 10 adenocarcinoma (Ad), 17 squamous cell carcinoma (Sq) and 12 large cell carcinoma (La)] by real-time polymerase chain reaction (PCR) with human 15254739 NA
gclc squamous-cell carcinoma We examined the gene expression of GCLM and GCLC in 39 xenografts of human non-small cell lung cancer [NSCLC; 10 adenocarcinoma (Ad), 17 squamous cell carcinoma (Sq) and 12 large cell carcinoma (La)] by real-time polymerase chain reaction (PCR) with human 15254739 NA
gclc large-cell carcinoma We examined the gene expression of GCLM and GCLC in 39 xenografts of human non-small cell lung cancer [NSCLC; 10 adenocarcinoma (Ad), 17 squamous cell carcinoma (Sq) and 12 large cell carcinoma (La)] by real-time polymerase chain reaction (PCR) with human 15254739 NA
gclc adenocarcinoma Squamous cell carcinoma xenografts significantly showed higher expression of GCLM gene than adenocarcinoma xenografts (p=0.023, t-test), while there was no significant difference in GCLC gene expression levels between each histopathological xenograft. 15254739 Human
gclc squamous-cell carcinoma Squamous cell carcinoma xenografts significantly showed higher expression of GCLM gene than adenocarcinoma xenografts (p=0.023, t-test), while there was no significant difference in GCLC gene expression levels between each histopathological xenograft. 15254739 Human
gclc hepatoma To determine whether increased GSH biosynthetic capacity enhances cellular resistance to tumor necrosis factor-alpha- (TNF-alpha-) induced apoptotic cell death, we have established several mouse liver hepatoma (Hepa-1) cell lines overexpressing GCLC and/o 15288121 Mouse
gcs nsclc METHODS: Eighty-five samples of NSCLC were studied using immunohistochemistry with polyclonal antibodies to the heavy and light subunits of gamma GCS (gamma GCS-h, gamma GCS-l), and the expressions were correlated with apoptosis and patients survival. 11753966 NA
gcs nsclc There was a strong association between the expression of the heavy and light subunits of gamma GCS in NSCLC (P = 0.003). 11753966 Human
gcs nsclc Because expression of gamma GCS has been connected with chemoresistance, downregulation of its activity by inhibitors in NSCLC might have putative therapeutic potential in the treatment of lung carcinoma. 11753966 Human
gcs malignant tumors However, the expression of this enzyme has not been systematically investigated in malignant tumors, and there are no studies of gamma GCS in biopsy specimens of malignant mesothelioma. 12196927 Human
gcs rb There was reverse correlation between P16 and Rb expression in 90 GCs (P<0.05). 15818729 Human
glcl breast carcinomas AIMS: To investigate the immunohistochemical expression of the catalytic and regulatory subunits of gamma-glutamyl cysteine synthetase, i.e. glutamate-L-cysteine ligase (GLCL) in 274 invasive and in-situ breast carcinomas. 14764056 NA
glcl tumour METHODS AND RESULTS: In the tumour cells GLCL reactivity was observed in 50% and 44% of the cases for the catalytic and the regulatory subunits, respectively. 14764056 Human
glcl breast carcinomas CONCLUSIONS: Expression of the catalytic and regulatory subunits of GLCL is found in a substantial number of breast carcinomas and their expression is more pronounced in lobular invasive and in-situ carcinomas. 14764056 Human
glcl in-situ carcinomas CONCLUSIONS: Expression of the catalytic and regulatory subunits of GLCL is found in a substantial number of breast carcinomas and their expression is more pronounced in lobular invasive and in-situ carcinomas. 14764056 Human
gamma-glutamylcysteine synthetase pd In contrast, the activity of the synthetic enzyme, gamma-glutamylcysteine synthetase, was unaltered in SN and other brain areas in PD. 8080243 Human
gamma-glutamylcysteine synthetase pd Similarly, glutathione peroxidase and glutathione transferase activities were unaltered in SN or in other brain regions in PD. gamma-Glutamylcysteine synthetase, gamma-glutamyltranspeptidase, glutathione peroxidase, and glutathione transferase activities 8080243 Human
gamma-glutamylcysteine synthetase leukemia METHODS: Leukemia (NB4, HL60, U937) and lymphoma (Jurkat and Namalwa) cell lines were treated with As2O3 together with buthionine sulfoximine (BSO), a selective inhibitor of gamma-glutamylcysteine synthetase. 11776809 NA
gamma-glutamylcysteine synthetase lymphoma METHODS: Leukemia (NB4, HL60, U937) and lymphoma (Jurkat and Namalwa) cell lines were treated with As2O3 together with buthionine sulfoximine (BSO), a selective inhibitor of gamma-glutamylcysteine synthetase. 11776809 NA
gamma-glutamylcysteine synthetase ovarian serous adenocarcinoma (1) Comparison of gene expression between CDDP-sensitive human ovarian serous adenocarcinoma cell lines and CDDP-resistant cell lines revealed that gamma-glutamylcysteine synthetase, glutathione peroxidase-like protein, dehydrogenase (UGDH), NAD(P)H: quin 11925926 Human
gamma-glutamylcysteine synthetase cancer Gamma-glutamylcysteine synthetase (gammaGCS), a rate-limiting enzyme in glutathione biosynthesis, plays a central role in glutathione homeostasis and is a target for development of potential therapeutic agents against parasites and cancer. 15477603 Human
gamma-glutamylcysteine synthetase lung cancers Using lung epithelial cells, the progenitor cells of lung cancers, we report that crocidolite asbestos initially depletes intracellular glutathione followed by up-regulation of both catalytic and modifier subunits of gamma-glutamylcysteine synthetase. 15520183 Human
gamma-glutamylcysteine synthetase canine mammary tumor Selenite addition increased the activity of gamma-glutamylcysteine synthetase in canine mammary tumor cell line 11 and non-neoplastic canine mammary cells, but not in canine mammary tumor cell line 13 cells. 15539239 Human
gamma-glutamylcysteine synthetase small-cell lung cancer We previously demonstrated that reduced intracellular redox conditions could be achieved in stably transfected small cell lung cancer cells with gamma-glutamylcysteine synthetase (gamma-GCSh) cDNA which encodes a rate-limiting enzyme in the biosynthesis o 16032782 Human

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